MicroRNA amplification and detection technologies: …
WEBDec 12, 2018 · MicroRNAs (miRNAs) are evolutionary conserved, ~18–24 nucleotides long non-coding RNA, playing a significant role in controlling human gene expression by post-transcriptional gene regulation or...
MicroRNA profiling: approaches and considerations - Nature
WEBApr 18, 2012 · MicroRNAs (miRNAs) are small RNAs (∼ 22 nucleotides long) that post-transcriptionally regulate the expression of thousands of genes in a broad range of organisms.
microRNAs in action: biogenesis, function and regulation
WEBJun 28, 2023 · The authors summarize our current understanding of miRNA biogenesis, function and processing, and highlight challenges to address in future research.
Bringing MicroRNAs to Light: Methods for MicroRNA …
WEBJan 18, 2021 · MiRNA transcripts are matured from pri-miRNA over pre-miRNA to mature miRNA, a process that includes multiple steps and enzymes. Many tools are available to identify and quantify specific miRNAs, ranging from measuring total miRNA, specific miRNA activity, miRNA arrays and miRNA localization.
MicroRNA profiling: approaches and considerations - PMC
WEBMicroRNA expression profiling has proven to be useful for identifying miRNAs that are important in regulation of a range of processes, including organismal development and establishment and maintenance of tissue differentiation.
Measurements Methods for the Development of MicroRNA …
WEBJan 25, 2021 · 3.3. miRNA Microarrays. Microarrays represent a high-throughput method used to concurrently detect presence and changes in levels of a wide range of miRNAs in an individual experiment. Microarrays are based on nucleic acid hybridization between target molecules and their corresponding complementary probes.
Profiling microRNA expression with microarrays - ScienceDirect
WEBFeb 1, 2008 · miRNA microarrays have been successfully used for the primary identification of new miRNAs that were predicted by bioinformatics approaches, for the dissection of differential expression of different miRNAs in the same cells, and for the comparison of miRNA expression profiles from different tissues or cells.
Quality Assessment and Data Analysis for microRNA Expression …
WEBDec 22, 2008 · MicroRNAs are small (∼22 nt) RNAs that regulate gene expression and play important roles in both normal and disease physiology. The use of microarrays for global characterization of microRNA expression is becoming increasingly popular and has the potential to be a widely used and valuable research tool.
MicroRNA detection by microarray | Analytical and ... - Springer
WEBJan 9, 2009 · The detection of miRNA expression is a very important first step in miRNA exploration. Several methodologies, including cloning, northern blotting, real-time RT-PCR, microRNA arrays and ISH (in situ hybridization), have been developed and applied successfully in miRNA profiling.
Frontiers | Bringing MicroRNAs to Light: Methods for MicroRNA ...
WEBJan 17, 2021 · Microarrays can compare levels of miRNA expression in different organs, tissues and species, and are a major, high throughput tool to analyze hundreds of miRNAs simultaneously. Sensitivity of microarrays is relatively low, mostly due to the amount of miRNA applied to the array.