The Iroquois religious beliefs are centered on an omniscient ‘Great Spirit’, who they believe is also their creator. They are strong proponents of anthropomorphism or animated nature and seasons. Many Iroquois are followers of Christianity. They show great respect and reverence at the mention of Handsome Lake, the Iroquois prophet.

The elaborate religious cosmology of the Iroquois was based on an origin tradition in which a woman fell from the sky; other parts of the religious tradition featured deluge and earth-diver motifs, supernatural aggression and cruelty, sorcery, torture, cannibalism, star myths, and journeys to the otherworld.

The religion of the Iroquois, also known as the Haudenosaunee Confederacy, was a central aspect of their culture and society. It played a significant role in shaping their worldview, values, and practices.

The Iroquois believed in many spirits, including the spirits of the three sisters, who were responsible for harvesting corn, beans, and squash. Iroquois spirituality also included belief in the Thunderer and the Great Spirit.