MicroRNA profiling: approaches and considerations - Nature
WEBApr 18, 2012 · miRNA expression profiling is useful for identifying miRNAs that are important in the regulation of a range of processes, including organismal development, tissue differentiation and disease...
MicroRNA profiling: approaches and considerations - PMC
WEBHere we define miRNA profiling as measurement of the relative abundance of a cohort of miRNAs, ranging from a group of several miRNAs of specific biological interest, to comprehensive profiling of all miRNAs in a given species (typically numbering in …
Small RNA-Sequencing: Approaches and Considerations for miRNA …
WEBMay 27, 2021 · Small RNA-seq enables genome-wide profiling and analysis of known, as well as novel, miRNA variants. Moreover, its high sensitivity allows for profiling of low input samples such as liquid biopsies, which have now found applications in …
MicroRNA profiling: approaches and considerations - PubMed
WEBApr 18, 2012 · Technological advances have spawned a multitude of platforms for miRNA profiling, and an understanding of the strengths and pitfalls of different approaches can aid in their effective use. Here, we review the major considerations for carrying out and interpreting results of miRNA-profiling studies.
An integrated expression atlas of miRNAs and their promoters
WEBAug 21, 2017 · Matched miRNA and CAGE expression profiles. In FANTOM5, a large collection of human and mouse primary cell types, cell lines, and tissues was profiled by CAGE to identify mRNA and long...
microRNA Expression Profiling: Technologies, Insights, and …
WEBAs a class of ~22 nt long small noncoding RNAs, miRNAs present unique challenges in expression profiling that require careful experimental design and data analyses. We will particularly discuss how normalization and the presence of miRNA isoforms can impact data interpretation.
WEBHere we define miRNA profiling as the measurement of the relative abundance of a cohort of miRNAs, ranging from a group of several miRNAs of specific biologi-cal interest to...
MicroRNA Profiling: Methods and Protocols | SpringerLink
WEBChapters detail miRNA biogenesis, isolating RNA, extracellular vesicles (EVs), circulating miRNAs, analyzing miRNA and miRDeep-P2, protocols for total RNA isolation from cells, cell-derived products, isolation and characterization of exosomes, serum, plasma specimens, and software tools.
MicroRNA (miRNA) Profiling - SpringerLink
WEBHowever, miRNA expression profiling is essential for the investigation of the biological functions and clinical applications of miRNAs. Therefore, in this chapter, we review and discuss commonly used techniques for miRNAs profiling, as …
Bringing MicroRNAs to Light: Methods for MicroRNA …
WEBJan 18, 2021 · Many tools are available to identify and quantify specific miRNAs, ranging from measuring total miRNA, specific miRNA activity, miRNA arrays and miRNA localization. The various miRNA assays differ in accuracy, cost, efficiency and convenience of monitoring miRNA dynamics.